Table 2. Kinetic parameters for CAD homologues AtCAD2-5, 7, and 8.
| Substrate | Km, μM | Vmax, pKat/μg | kcat, sec-1 | kenz, M-1·sec-1 | |
|---|---|---|---|---|---|
| AtCAD4 | 1 | 47 | 44.0 | 3.44 | 74,000 |
| 2 | 87 | 17.1 | 1.33 | 15,000 | |
| 3 | 65 | 26.8 | 2.10 | 32,000 | |
| 4 | 85 | 32.3 | 2.52 | 30,000 | |
| 5 | 274 | 9.2 | 0.72 | 2,600 | |
| AtCAD5 | 1 | 13 | 187.3 | 14.52 | 1,091,000 |
| 2 | 68 | 94.1 | 7.30 | 107,000 | |
| 3 | 35 | 157.4 | 12.19 | 348,000 | |
| 4 | 22 | 106.9 | 8.28 | 370,000 | |
| 5 | 20 | 177.0 | 13.72 | 700,000 | |
| AtCAD2 | 1 | 114 | 3.3 | 0.27 | 2,400 |
| 2 | 161 | 22.2 | 1.81 | 11,000 | |
| 3 | 452 | 8.0 | 0.65 | 1,400 | |
| 4 | 336 | 16.4 | 1.34 | 4,000 | |
| 5 | 2,161 | 48.1 | 3.93 | 1,800 | |
| AtCAD3 | 1 | 292 | 20.1 | 1.65 | 5,600 |
| 2 | 581 | 9.7 | 0.80 | 1,400 | |
| 3 | 362 | 4.8 | 0.39 | 1,100 | |
| 4 | 534 | 17.9 | 1.47 | 2,800 | |
| 5 | 629 | 9.1 | 0.74 | 1,200 | |
| AtCAD7 | 1 | 320 | 28.6 | 2.18 | 6,800 |
| 2 | 3,685 | 79.0 | 6.04 | 1,600 | |
| 3 | 675 | 13.0 | 0.99 | 1,500 | |
| 4 | 756 | 2.7 | 0.21 | 280 | |
| 5 | 313 | 0.6 | 0.05 | 150 | |
| AtCAD8 | 1 | 302 | 20.4 | 1.59 | 5,300 |
| 2 | 683 | 7.0 | 0.54 | 800 | |
| 3 | 141 | 5.6 | 0.44 | 3,100 | |
| 4 | 457 | 10.4 | 0.81 | 1,800 | |
| 5 | 898 | 28.9 | 2.25 | 2,500 |