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Mutation of several acidic residues in the first and second ECLs of CXCR3 significantly increases affinity for CXCL11 but not for CXCL10. The relative abilities of increasing concentrations of unlabelled ligand to displace 0.1 nM 125I-CXCL11 (A) or 0.1 nM 125I-CXCL10 (B) in homologous competition assays using CXCR3 transfectants.
