Table 1.

Expression, chemotaxis and binding properties of CXCR3 mutants

Construct	% of WT CXCR3 surface expression	% of WT chemotaxis to 30 nM CXCL11	% of WT chemotaxis to 30 nM CXCL10	KD CXCL11 binding (nM)	KD CXCL10 binding (nM)
WT CXCR3	100	100	100	10.9 ± 5.6	4.7 ± 0.5
D112N	76.8 ± 7.6	80.2 ± 24.5	17.8 ± 1.5***	3.8 ± 0.7	ND
D195N	74.2 ± 2.1	90.3 ± 19.7	14.7 ± 3.9**	16.0 ± 3.4	4.7 ± 1.1
E196Q	188.0 ± 79.9	76.0 ± 18.9	9.5 ± 4.1**	0.4 ± 0.1	7.6 ± 3.4
D278N	92.6 ± 1.4	80.7 ± 27.6	49.3 ± 5.9*	2.6 ± 1.0	30.9 ± 3.2*
D282N	62.1 ± 26.9	87.5 ± 17.5	84.2 ± 18.9	1.4 ± 0.2	2.9 ± 1.3
E293Q	134.8 ± 16.8	77.6 ± 23.1	49.8 ± 4.4**	3.7 ± 0.9	1.2 ± 0.0*
D297N	90.2 ± 10.4	44.1 ± 6.1*	72.9 ± 25.7	26.3 ± 0.5*	7.1 ± 0.5*

Surface expression and chemotaxis are expressed relative to values obtained for transfectants expressing WT CXCR3. ND indicates not determined. The mean values ± SEM from at least three experiments are shown. ^***P < 0.001, ^**P < 0.01 and ^*P < 0.05, significantly different from corresponding values for the WT CXCR3; Student's t-test.