Figure 7.

The effects of the Rho inhibitor, C3 toxin, on PGE₂-stimulated Nurr1 protein expression (A) and the phosphorylation of I-κBα (B) in HEK cells stably transfected with the human EP₁ receptor (HEK-EP1). (A, B) HEK-EP1 cells were pretreated with either vehicle (control) or 5 µM C3 toxin overnight and were then incubated with either vehicle (veh) or 1 µM PGE₂ for 3 h at 37°C. Lysates were prepared and subjected to immunoblot analysis with antibodies against either Nurr1, phospho-I-κBα (p-I-κBα) or vinculin. Representative immunoblots are shown that were repeated at least three times for each antibody and condition.