Fig. 2.

Fast cleavage of RS19. (A) Time course of self-cleavage of RS19. (Inset) Cleavage reactions were performed over a range of concentrations of MgCl₂, and the apparent first-order rate constant was plotted as a function of MgCl₂ concentration. (B) Time course of self-cleavage of mutants of G11 (see Fig. 1B): G11, •; G11L, ○; AN16, ♦; AN16ΔIa, ⋄; AN16εL, ▵; and AN16ε, ▴. (Inset) The burst phase of AN16ε cleavage. (C) Time course of self-cleavage of RS19 and 3′ permutations (see Fig. 1C): RS19, •; RS19ΔIa, ⋄; RS19Δ5′α, ♦; RS19Δ5′γ, ▵; and RS19ΔL, ▴.(Inset) The burst phase of RS19ΔL cleavage. (B and C Insets) The x axis is time (s), and the y axis is fraction cleaved. The lines show the nonlinear least-squares fittoa first-order rate equation. Kinetic parameters are summarized in Table 1.