Table 1. Estimated kinetic parameters (mean ± standard deviation).

	Extra nt in cleavage site loop	Helix la	kobs, s-1	fmax	k1, s-1	k-1, s-1
3′ permutations
RS19	CUAA	+	3.0 ± 0.19	0.89 ± 0.023	≥2.7 ± 0.20	≤0.32 ± 0.065
RS19Δ5′	CUAA	+	4.2 ± 0.25	0.91 ± 0.015	≥3.8 ± 0.24	≤0.37 ± 0.060
RS19Δla	—	-	0.33 ± 0.036	0.91 ± 0.011	≥0.31 ± 0.029	≤0.029 ± 0.0071
RS19Δ5′α	CUAA	+	2.0 ± 0.22	0.77 ± 0.010	≥1.6 ± 0.20	≤0.45 ± 0.027
RS19β	CUAA	+	1.7 ± 0.24	0.85 ± 0.029	≥1.5 ± 0.16	≤0.27 ± 0.082
RS19γ	CUAA	+	1.8 ± 0.13	0.87 ± 0.015	≥1.6 ± 0.084	≤0.24 ± 0.044
RS19Δ5′γ	CUAA	+	2.9 ± 0.25	0.87 ± 0.017	≥2.5 ± 0.27	≤0.38 ± 0.016
RS19ΔL	—	+	1.9 ± 0.38	0.11 ± 0.0055	0.21 ± 0.031	1.7 ± 0.35
RS19ΔL (Ch)	—	+	1.9 ± 0.42*	0.12 ± 0.0049*	0.22 ± 0.049	1.7 ± 0.37
RS19L5	CUAAA	+	3.7	0.88	≥3.3	≤0.41
RS19L4a	CAAA	+	2.1	0.86	≥1.8	≤0.28
RS19L4b	AUAA	+	2.4	0.86	≥2.1	≤0.33
RS19L4c	AAAA	+	2.8	0.90	≥2.5	≤0.26
RS19L3	AAA	+	1.0	0.88	≥0.93	≤0.12
RS19L2	AA	+	0.63	0.82	≥0.51	≤0.11
RS19L1	A	+	10 ± 1.6	0.26 ± 0.012	2.6 ± 0.46	7.4 ± 1.2
RS19L1 (Ch)	A	+	6.2 ± 1.4*	0.32 ± 0.03*	2.0 ± 0.51	4.2 ± 0.95
5′ permutations
G11	—	+	0.0021 ± 0.00031	0.91 ± 0.0030	n/a	n/a
G11L	CUAA	+	0.14 ± 0.0045	0.94 ± 0.046	n/a	n/a
AN16	—	+	0.11 ± 0.010	0.93 ± 0.012	n/a	n/a
AN16Δla	—	-	0.31 ± 0.016	0.91 ± 0.038	n/a	n/a
AN16εL	CUAA	+	2.2 ± 0.18	0.86 ± 0.014	≥1.9 ± 0.13	≤0.33 ± 0.050
AN16ε	—	+	1.9 ± 0.21	0.15 ± 0.0045	≥0.24 ± 0.047	≤1.6 ± 0.17

n/a, Not available.

^*Calculated first-order parameters of burst phase; f_max = k₁/(k₁ + k_-1); k_obs = k₁ + k_-1