Figure 2.

P2 receptors gene expression and effects of P2 receptor antagonists on [³H]-thymidine incorporation. (A, B) Images of RT-PCR and Western blots for detecting mRNAs and proteins of P2 receptors in human cardiac fibroblasts. (C) [³H]-thymidine incorporation was determined in cells incubated with ATP (100 µM), AMP-CPP (α,β-methylene ATP, 100 µM) or ATP-γS (100 µM) for 24 h. (D) Pre-incubation (for 30 min) with reactive blue-2 (RB-2, 1 µM) or suramin (10 µM) attenuated or abolished the increase in [³H]-thymidine incorporation rate induced by ATP. n= 4, *P < 0.05, **P < 0.01 vs. control; ^#P < 0.05, ^##P < 0.01 vs. ATP alone.