Figure 7.

Effect of silencing the P2 receptors on ATP-stimulated proliferation of human cardiac fibroblasts. (A) Western blots and mean values of protein expression of P2X₄, P2X₇ and P2Y₂ in cells treated with corresponding siRNA molecules (n= 4, *P < 0.05, **P < 0.01 vs. Lipofectamine or control siRNA). (B) Cell proliferation in cells treated with siRNA molecules (40 nM) targeting P2X₄, P2X₇ and P2Y₂ (n= 3). (C) [³H]-thymidine incorporation in cells treated with siRNA molecules (40 nM) targeting P2X₄, P2X₇ and P2Y₂, respectively (n= 3). **P < 0.01 vs. control siRNA without ATP; ^#P < 0.05, ^##P < 0.01 vs. control siRNA with ATP.