Figure 2.

HO-1 and BDNF mediate the neuroprotective effect of CAPE. (A, B) Effect of an HO-1 inhibitor ZnPPIX on the protective effect of CAPE on dopaminergic neurons (A) and nitrite concentration in the medium (B). ZnPPIX and CAPE at indicated concentrations were concomitantly applied with 10 µg·mL⁻¹ LPS for 72 h following 24 h treatment with 50 ng·mL⁻¹ IFN-γ. Number of slices examined for each condition is 28–30. Values of nitrite concentrations are of 6 wells from five independent experiments. (C, D) Effect of anti-BDNF neutralizing antibody on the protective effect of CAPE on dopaminergic neurons (C) and nitrite concentration in the medium (D). Anti-BDNF (5 µg·mL⁻¹), and ZnPPIX and CAPE at the indicated concentrations were applied with 10 µg·mL⁻¹ LPS for 72 h following 24 h treatment with 50 ng·mL⁻¹ IFN-γ. Number of slices examined for each condition is 27–30. Values of nitrite concentrations are of 6 wells from five independent experiments. ***P < 0.001 versus control; †P < 0.05, ††P < 0.01, †††P < 0.001 (anova results: for panel A, F(7,225) = 23.45, P < 0.0001; for panel B, F(7,40) = 107.39, P < 0.0001; for panel C, F(7,228) = 34.12, P < 0.0001; for panel D, F(7,40) = 126.92, P < 0.0001).