Fig. 2.
Electrophoretic mobility-shift assay complexes in extracts of WT and dOct cells. Nuclear extracts (10 μg) from WT, dOct, retrovirus control (C), and retrovirus rOct cells were mixed with HSV TAATGARAT probe DNA (ICP0, pRB608) in the absence (Left) or presence (Right) of exogenously added VP16 protein (50 ng). Complexes were resolved in 4% native acrylamide gels and visualized by autoradiography. Oct-1, Oct-1/DNA complex; EC, HSV IE enhancer core complex containing Oct-1, VP16, and HCF-1.