Skip to main content
. 2012 Jun;166(4):1261–1271. doi: 10.1111/j.1476-5381.2012.01848.x

Figure 1.

Figure 1

Intra-articular administration of exogenous CGRP causes a dose-dependent increase in the endothelial cell proliferation index in the synovium. (A). PCNA indices in saline-injected controls and 24 h after intra-articular injection of CGRP from 0.025 to 2.5 nmol per knee. The two higher doses of CGRP caused an increase in the PCNA index (P < 0.01 and P < 0.001, respectively) compared with saline-injected controls. Data shown are means ± SD; n = 6 rats per group. (B). Main panel: Synovium from a rat 24 h after intra-articular injection of 2.5 nmol CGRP, showing CD31-positive vascular cells and PCNA-positive proliferating nuclei. Counterstaining with DAPI shows all other nonstained nuclei. Arrows indicate PCNA-positive endothelial cells. This allows determination of the PCNA index as the percentage of vascular nuclei, which were positive for the proliferation marker. Inset shows a blood vessel from a control saline-injected synovium not displaying PCNA-positive endothelial cell nuclei. Bar = 100 µm.