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. 2012 Jun;166(4):1261–1271. doi: 10.1111/j.1476-5381.2012.01848.x

Figure 5.

Figure 5

Components of the CGRP and adrenomedullin receptor-ligand complex are expressed in rat and human synovia. (A). Products of RT-PCR for GAPDH, adrenomedullin (AdM), CRLR, RAMP-1, RAMP-2 and RAMP-3 are shown after separation on agarose gels. Samples from synovia taken 24 h after intra-articular injection with capsaicin (C1–C6, lanes 1–6) and naïve knees (N1–N6, lanes 7–12) were analysed. Positive controls (+, lane 13) were rat brain cDNA. Negative controls (–, lane 14) were PCR's without any template DNA. (B). Examples of PCR products from RT-PCR on human synovia for CRLR, RAMP-1, RAMP-2, RAMP-3 and adrenomedullin are shown after separation on agarose gels. Samples from OA without inflammation (OA(n), lanes 1, 2), severely inflamed OA (OA(i), lanes 3, 4), normal post mortem (PM; lanes 5, 6) and RA (lane 7) are shown. Positive controls (+, lane 9) were HUVEC cells for CRLR and DU145 cells for RAMPs-1, -2 and -3. Negative controls (–, lane 10) were PCRs without any template DNA. (C). Table showing detection rates for PCR products in human synovia.