Fig. 2.

(A) Selected ¹³C–¹H strips from the ¹³C-edited/¹²C-filtered 3D NOESY spectrum (21) identifying NOEs at the dimer interface of ThKaiA180C. The spectrum was acquired in a heterodimeric sample of unenriched and ¹³C/¹⁵N-enriched protein. (B) The dimer interface of ThKaiA180C. Shown here is α₄ of one subunit in magenta, and in blue the C-terminal region of α₂′, the loop between α₂′ and α₃′, and the N-terminal region of α₃′ and α₄′ are shown. Residues identified as being part of the dimer interface by the ¹³C-edited/¹²C-filtered 3D NOESY and the intersubunit disulfide bond and salt-bridge are also shown.