Figure 7.

Effect of NS8593 on the viability of HEK 293 cells overexpressing recombinant TRPM7. (A) Tetracyclin(Tet)-induced (1 µM, 18 h) expression of the human TRPM7 protein as assessed by immunofluorescence staining using anti-TRPM7 antibody. Scale bars – 10 µm. (B) Representative I–V relationship of fully developed whole-cell TRPM7 currents measured in stimulated HEK 293 cells (1 µM tetracycline, 18–24 h). (C) Effect of NS8593 (10 µM) on the morphology of tetracycline-induced (1 µM, 36 h) HEK 293 cells. Scale bars – 50 µm. (D) Viability of HEK 293 cells expressing TRPM7 in the presence of different concentrations of NS8593. Experiments were performed as in (C), and the total number of viable cells was compared with that of unstimulated cells (– tetracycline). Data from three independent experiments are shown. ***P≤ 0.001; n.s., not significantly different (t-test).