Figure 9.

Inhibition of native TRPM7-like currents in vascular smooth muscle cells, podocytes and ventricular myocytes by NS8593. Representative current density–voltage relationships of native whole-cell currents (MIC) induced by depletion of internal Mg²⁺ in mouse smooth muscle cells freshly isolated from brain arteries (A), primary mouse podocytes (B) and primary human ventricular myocytes (C). Measurements were performed as in Figure 2A. The insert shows corresponding current densities at +100 mV acquired before and during current induction or in the presence of NS8593. The insert in (A) shows recovered currents, if pretreated cells were reperfused with NS8593-free solution. Numbers above columns indicate the number of cells measured; ***P≤ 0.001; **P≤ 0.01; *P≤ 0.05 (t-test).