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. 2012 Jun;166(4):1357–1376. doi: 10.1111/j.1476-5381.2012.01855.x

Figure 10.

Figure 10

Effects of NS8593 on motility and proliferation of HEK 293 cells. (A) Images of living HEK 293 cells cultured without or with indicated concentrations of NS8593 (24 h). In the presence of 30 µM NS8593, cell colonies frequently displayed a tight and round shape. Insert illustrates at a higher magnification the colony indicated by the red arrow. Scale bars – 30 µm. (B, C) Effect of 30 µM NS8593 on cell motility as assessed by a wound healing assay. (B) Representative images of HEK 293 cells acquired either immediately or 24 h after wounding. Outline of the wound and percentage of the closure area (%) were determined using ImageJ. Scale bars – 200 µm. (C) Data from six independent assays are shown; **P≤ 0.01 (t-test). (D) Viability of HEK 293 cells cultured (48 h) in the presence of indicated concentrations of NS8593. The total number of viable cells was compared with that of untreated cells (100%). Data from three independent experiments are shown, *P≤ 0.05; n.s., not significantly different (t-test).