Figure 10.
Electrically-induced contractions in the mouse isolated ileum of (A,C) control and (B,D) croton oil-treated mice: inhibitory effect of CBC (10−8–10−4 M) alone (vehicle) or in the presence of ω-conotoxin (10−8 M) (A,B), IBMX (10−7 M), rolipram (10−6 M) or forskolin (10−7 M) (C,D). Each point represents mean ± SEM of 7–8 experiments. Both in control mice (A) and in croton oil-treated mice (B), ω-conotoxin (but not IBMX, rolipram or forskolin) significantly (P < 0.01) reduced the inhibitory effect of CBC (significant difference between curves). The insert in (C) shows that IBMX (10−7 M), rolipram (10−6 M) and forskolin (10−7 M) significantly (P < 0.01) reduced the inhibitory effect of WIN55,212-2 (10−9–10−6 M) on electrically induced contractions in the ileum of control mice (n= 5).