Inhibition of the peak amplitude of contractions evoked by UDP and UTP. The mean peak amplitude of contractions of endothelium-denuded rat isolated IPA evoked by (A) UDP (300 µM) and (B) UTP (300 µM) in the presence of niflumic acid (1 µM) (NFA), nifedipine (1 µM) (nifed), niflumic acid (1 µM) plus nifedipine (1 µM) (NFA + nifed), DIDS (100 µM), DIDS (100 µM) plus nifedipine (1 µM) (DIDS + nifed) and in Ca2+-free buffer (0[Ca]ext), expressed as a percentage of control responses, is shown. The numbers in parentheses show n for each. Vertical lines show SEM. *P < 0.05, **P < 0.01, ***P < 0.001 for responses after treatment compared with control. #P < 0.05 for responses in Ca2+-free buffer compared with the other treatments.