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. 2012 Mar 1;2:21. doi: 10.3389/fcimb.2012.00021

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Copyright © 2012 Han, Wang, Sahin, Shen, Guo, Shen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Supercoiling activities of the recombinant WT gyrase and the three recombinant mutant gyrases measured by an in vitro assay. Lane 1, DNA ladder; Lane 2, relaxed pBR322 (control); Lane 3, WT GyrA + GyrB; Lane 4, mutant GyrA (Thr-86-Ile) + GryB; Lane 5, mutant GyrA (Thr-86-Lys) + GryB; Lane 6, mutant GyrA (Asp-90-Asn) + GryB; and Lane 7, supercoiled pBR322 (control). SC indicates supercoiled DNA and R represents relaxed DNA.