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. 2012 Mar 1;2:21. doi: 10.3389/fcimb.2012.00021

Figure 3.

Figure 3

Agarose gel electrophoresis analysis of plasmid topoisomers extracted from different strain background. The plasmid DNA was run on a 1.0% agarose gel containing 20 μg/ml chloroquine. (A) Plasmid pRY107 isolated from strain S3B derivatives. Lane 1, pRY107 from 62301S; Lane 2, pRY107 from 62301R (carrying the Thr-86-Ile mutation in GyrA); Lane 3, pRY107 from 52901-II2 (carrying the Thr-86-Lys mutation in GyrA); and Lane 4, pRY107 from 62301R37 (carrying the Asp-90-Asn mutation in GyrA). (B) Plasmid pRY107 isolated from strain 11168 and its derivatives. Lane 1, pRY107 from NCTC 11168; and Lane 2, pRY107 from 11168CT (carrying the Thr-86-Ile mutation in GyrA). The results of densitometric scanning are shown to the right of each gel image. The numbers on the left of the densitometric scanning correspond to the lane numbers of the gel image. The number below each lane indicates the percentage of the most supercoiled DNA in the total population of plasmid topoisomers as measured by densitometry.