Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 1;2:21. doi: 10.3389/fcimb.2012.00021

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Han, Wang, Sahin, Shen, Guo, Shen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

PMC Copyright notice

Agarose gel electrophoresis showing the inhibition of DNA gyrase supercoiling activities by ciprofloxacin. In each reaction, the relaxed pBR322 was incubated with different enzymes in the presence of various concentration of ciprofloxacin. (A) Reactions with recombinant GyrA from the WT strain and GyrB; (B) reactions with mutant GyrA (Thr-86-Lys) and GyrB; (C) reactions with mutant GyrA (Asp-90-Asn) and GyrB; and (D) reactions with mutant GyrA (Thr-86-Ile) + GyrB. The numbers on top of each panel are the concentrations of ciprofloxacin (μg/ml) used in the reaction. SC indicates supercoiled DNA and R represents relaxed DNA (TopoGen). In each lane, the most supercoiled band migrates faster than the other topoisomers.