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. 2012 May;166(2):721–736. doi: 10.1111/j.1476-5381.2011.01791.x

Figure 1.

Figure 1

Expression of β2-adrenoceptors in mouse mammary tumour cell line MC4-L5 and the human IBH-4, IBH-6 and MDA-MB-231 cells by immunofluorescence and RT-PCR. (A,B) Mouse mammary tumour cells MC4-L5. (C,D) Human breast cancer cells IBH-4. (E,F) Human breast cancer cells IBH-6. (G,H) Human breast cancer MDA-MB-231 cells. (A,C,E,G) β2-RA. (B,D,F,H) Negative controls incubated without the primary antibody. (A,B,E,F) Photographs taken with a Plan Apo 40X/0.95 oil objective and (C,D,E,F) with an Apo 60X/1.40 oil one. (I) RT-PCR for β2-adrenoceptors (β2-AR) in human IBH-4, IBH-6 and MDA-MB-231 cells. (J) RT-PCR for β2-adrenoceptors in mouse MC4-L5 cells. In every case GAPDH was used as a control for RNA integrity and the RT-PCR process. The negative control was performed in the absence of cDNA.