Figure 4.

Inhibition of VEGFR2 phosphorylation and intracellular signalling in response to VEGF-A in primary endothelial cells. Cells were treated for 7.5 min with VEGF-A (25 ng·mL⁻¹) in the presence of (A) JK-P3 or (C) JK-P5 at between 10 nM and 10 µM concentration and processed for immunoblotting using anti-phospho VEGFR2 (Y1175), anti-VEGFR2, anti-phospho-PLCγ1 (Y783), anti-PLCγ1, anti-phospho-Akt (S473), anti-Akt, anti-phospho-ERK1/2 (T202/Y204), anti-ERK1/2 and anti-alpha-tubulin antibodies. (B) Quantification of VEGFR2 inhibition by JK-P3. (D) Quantification of VEGFR2 inhibition by JK-P5. Data are presented as mean ± SEM (n= 3). Significant inhibition of VEGFR2 phosphorylation denoted by ***P < 0.001 using anova.