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. 2012 May;166(2):774–787. doi: 10.1111/j.1476-5381.2011.01814.x

Figure 1.

Figure 1

Membrane potential and Ca2+ responses in EC tubes. (A) Continuous 4 h record of Vm from one cell within an EC tube illustrating hyperpolarization to ACh (0.3 and 3 µM) followed by NS309 (0.01–30 µM) with washout following each peak hyperpolarization. The value for EK=−90 mV was calculated with [K+]o= 5 and [K+]i= 145 mM. (B) Peak hyperpolarization for indicated concentrations of NS309 (n= 5); ΔVm (mV) = resting Vm− peak response Vm. (C) Peak hyperpolarization for indicated concentrations of ACh (n= 9). ΔVm calculated as in B. (D) Peak [Ca2+]i responses for indicated concentrations of ACh (n= 5) using Fura-2 dye. ΔF340/F380= peak response F340/F380− resting F340/F380. Respective pEC50s indicated by vertical broken lines are: (B) 6.40 ± 0.06; (C) 6.88 ± 0.09; (D) 7.34 ± 0.04. †ΔVm for 1 µM NS309 ≍ ΔVm for 3 µM ACh. Summary data are means ± SE.