Figure 4.

Reversible inhibition of dye transfer by CBX and βGA. Representative images following propidium iodide microinjection (into cell indicated by arrow). (A) Propidium iodide dye (0.1% in 2 M KCl) was microinjected under control conditions during intracellular recording for 30 min. Note robust dye transfer to surrounding cells. (B) As described in A at a separate site of the same EC tube after 5 min exposure to 100 µM CBX that was maintained during 30 min intracellular recording. Note absence of dye transfer. (C) As described in A at a separate site of the same EC tube following 20 min washout of 100 µM CBX with PSS. Note restoration of dye transfer. (D) As described in A. (E) As in B using 40 µM βGA instead of CBX. Note absence of dye transfer. (F) As described in C following 20 min washout of 40 µM βGA with PSS.