Figure 7.

Effect of GA derivatives on [Ca²⁺]_i responses to ACh. (A) Representative recording of EC [Ca²⁺]_i responses to 1 min superfusion of 3 µM ACh under control (C) conditions, following 10 min exposure to 10, 30 or 100 µM CBX, and following 20 min recovery (R) during washout of CBX. Vertical lines denote 10 min breaks in recording during superfusion with CBX in PSS at respective concentrations before ACh stimulation. (B) Summary data (n= 3) for the change in baseline F₃₄₀/F₃₈₀ ratio from resting control following 10 min superfusion with 10, 30 or 100 µM CBX and during recovery (R) following 20 min washout. (C) Summary data (n= 3) for response amplitude [= (maximal F₃₄₀/F₃₈₀ ratio during 1 min ACh) − (preceding baseline F₃₄₀/F₃₈₀ ratio)] under control conditions (C) and after 10 min exposure to respective [CBX]. (D–F) As in A, B and C using βGA instead of CBX. Note inhibition of EC [Ca²⁺]_i response to ACh with 100 µM βGA in F. *Significantly different from control, P < 0.05.