Figure 5.

Bacteria burden in murine tissue post immunization and challenge with S. aureus. Balb/c mice were passively immunized with CS-D7 (circles) or isotype control 20C2HA (squares) or PBS alone (triangles), and 2 h later were challenged via the tail vein with S. aureus SA025. At designated time points, subsets of animals were sacrificed and the blood and organs were harvested. Tissue bacterial burden was evaluated by homogenization of each organ in a total of 5 mL PBS and plating of 50 μL homogenate on TSA. The CFU count per 50 μL is plotted. For blood CFU determination, 10 μL of undiluted blood was plated. Data shown are means and ranges from mice (n = 4) in two experiments.