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Heme transfer from hemoglobin to heme minus IsdB (IsdB−) in the presence of mAbs.
| Sample number | Antibody added to IsdB− | Absorbance A400 (iron)/A280 (protein); (ratio) | |
|---|---|---|---|
| Starting sample (Antibody + IsdB−) | Final, total column fractions (Antibody + IsdB− + heme) | ||
| 1. | 20C2HA | 0.05/2.97 (0.02) | 0.80/3.55 (0.23) |
| 2. | CS-D7 | 0.03/2.64 (0.01) | 0.64/2.89 (0.22) |
| 3. | 2H2 | 0.06/2.84 (0.02) | 0.68/2.86 (0.24) |
