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IsdB interaction with immobilized human hemoglobin.
| No. | Sample | Starting sample A400/A280 (ratio) | Flow through A400/A280 | Eluted material A400/A280 | Total, post column fractions A400/280 (ratio) |
|---|---|---|---|---|---|
| 1. | IsdB− | 0.06/0.82(0.07) | 0.35/0.43 | 0.49/0.42 | 0.84/0.85(0.99) |
| 2. | IsdB+ | 0.82/0.95(0.87) | 0.59/0.50 | 0.58/0.38 | 1.18/0.88(1.34) |
