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. 2012 Apr 9;2:48. doi: 10.3389/fcimb.2012.00048

Figure 4.

Figure 4

(A) Illustration of yefM-yoeB and flanking DNA in CC30. yefM-yoeB are flanked by repeats LDR-1 and LDR-2 (black bars) in PT80/81 and ST30spa19 CA-MRSA, whereas HA-MRSA and contemporary MSSA have only one LDR and lack yefM-yoeB. (B) Nucleotide sequence of LDR-1 and LDR-2 from strain M1015, and the single LDR of MRSA 252. The lower-case gray shaded nucleotides differentiate LDR-1 and LDR-2. The single LDR of MRSA 252 is a hybrid of LDR-1 and LDR-2, which is suggestive of a recombination event. (C) Growth of S. aureus RN4220 harboring Pcad::yefM-yoeB or Pcad::yoeB, on BHI agar, or BHI supplemented with 5 μM cadmium, to induce Pcad.