Fig. 2.

Activation of TPKI/GSK3β, inactivation of PDH and inhibition Ach synthesis in rat septum cells cultured with amyloid β.¹⁶⁾ (A) Dependency of TPKI activation and PDH inactivation on the concentration of Aβ_1–42. The activity was measured after 12 hours incubation with various concentration of Aβ_1–42 shown in the abscissa. (B) Effect of Aβ_1–42 and Aβ_1–40 on the suppression of Ach synthesis. Intracellular level of Ach was determined by HPLC-electrochemical detector system after 12 hours culture with Aβ.