Figure 2.

MiR-155 induction can be mediated indirectly though secreted factors. (A) PBM were infected with FN at an MOI of 50 for 24 h. Media from infected cells was sterile filtered to remove bacteria and RNA was isolated from the cells (R and Fn). New PBM were cultured in the conditioned media for 24 h (CM R and CM FN) followed by RNA isolation. Mature miR-155 expression was assayed by qRT-PCR. (B) PBM were infected for 24 h, then the media from the infected cells was sterile filtered to generate conditioned media. New PBM were infected with F.n. at an MOI of 50 for 6 h or treated with conditioned media for 6 h in the presence of BAY-7085-11 (5 μM) or DMSO control. MiR-155 expression was assayed by qRT-PCR. These data are representative of at least three independent experiments. *Designates a p value < 0.05.