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. 2012 Jun 1;2:73. doi: 10.3389/fcimb.2012.00073

Figure 5.

Figure 5

Fos and Jun expression is highly up-regulated by infection in an NF-κB-dependent mechanism to control AP-1 activity. (A) PBM were pre-treated with DMSO or BAY-11-7085 (5 μM; designated as BAY in the figure) for 30 min then infected with F.n. at an MOI of 50 for 4 h. Cells were lysed and subject to western blotting for c-Fos, followed by actin re-probe. (B) Protein-matched lysates were probed for c-Jun and re-probed with actin antibody. (C) RAW264.7 macrophages were transfected with an AP-1 luciferase reporter. 14 h post-transfection cells were uninfected or infected with F.n. at an MOI of 50 for 1, 4, 8, or 24 h. Cells were lysed and luciferase activity was measured by a luminometer in triplicate. Data are represented as % increase over uninfected control. (D) RAW264.7 macrophages were transfected with the AP-1 reporter as in (C). Fourteen hours post-transfection cells were pre-treated with DMSO vehicle control, BAY-11-7085, or U0126 then infected for 8 h. Data are represented as % increase over uninfected samples. These data are representative of three independent experiments. *Designates a p value < 0.05.