Figure 6.

MiR-155 induction is dependent upon c-Fos. (A) RAW 264.7 macrophages were transfected with 4.0 μM siRNA control or siRNA against c-Fos. Thirty minutes post-transfection cells were left uninfected or infected with F.n. at an MOI of 50 for 4 h. Cells were then lysed and subject to western blotting for c-Fos. An antibody against Actin was used to re-probe the blots to ensure equal loading. (B) RAW 264.7 macrophages were transfected as done in part A and infected for 16 h. RNA was collected and miR-155 expression was assayed by qRT-PCR. These data are representative of three independent experiments. *Designates a p value < 0.05.