Figure 8.

MiR-155 induction by microbes/microbial ligands is NF-κB-dependent. (A–D) Human PBM were pre-treated with or without the NF-κB inhibitor (BAY-11-7085, designated as BAY in the figure), and subsequently treated with (A) LPS (500 ng/ml), (B) R848 (1 uM), or infected with (C) B. cenocepacia (Bc) at MOI 5, or (D) M. smegmatis (Ms) at MOI 5. mRNA was isolated from the cells 16 h later and assayed for miR-155 by qRT-PCR. These data are representative of six independent experiments. *Designates a p value < 0.05.