Figure 9.

c-Jun and c-Fos are induced by microbes/microbial ligands in an NF-κB-dependent manner. (A–D) Human PBM were pre-treated with or without the NF-κB inhibitor (BAY-11-7085, designated as BAY in the figure), and subsequently treated with (A) LPS (500 ng/ml), (B) R848 (1 uM), or infected with (C) B. cenocepacia (Bc) at MOI 5, or (D) M. smegmatis (Ms) at MOI 5. Cell lysates were collected 16 h later. Protein-matched lysates were analyzed by Western blotting with c-Jun or c-Fos antibodies. All membranes were re-probed with Actin antibody to ensure equal loading of protein in all lanes. These data are representative of four independent experiments.