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In vitro comparison of wildtype FRI1169 and variant JY3000 cytotoxicity. A549 cell cytotoxicity (A) and IL-8 response (B) after 4 h exposure to S. aureus broth culture supernates of FRI1169 (wildtype cytolytic) and JY3000 (biofilm-derived variant, non-cytolytic) grown for 7 or 14 h. *Indicates p < 0.05 compared to medium control (RPMI medium alone) by One-Way ANOVA, data plotted as mean ± standard deviation.
