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. 2012 Feb 21;2:18. doi: 10.3389/fcimb.2012.00018

Figure 2.

Figure 2

Characterization of exoprotein production by wildtype FRI1169 and variant JY3000. (A) 14 and 7 h broth culture supernates (20 μg of protein loaded per lane) were separated by SDS-PAGE and silver-stained. Media denotes lane electrophoresed with Todd Hewitt media alone. Arrow denotes small molecular weight (<10 kDa) band only present in FRI1169 cultures. (B) Total protein content measured in three separate overnight broth culture supernates from JY3000 and FRI1169 via Bio Rad reagent. (*P = 0.005 by Student's t-test). (C) Western immunoblot results showing differences in α-toxin amounts produced by wildtype FRI1169 and JY3000.