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. 2012 Feb 21;2:18. doi: 10.3389/fcimb.2012.00018

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Copyright © 2012 Spaulding, Satterwhite, Lin, Chuang-Smith, Frank, Merriman, Schaefers, Yarwood, Peterson and Schlievert.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Ethanol (80%) separation of exoprotein and determination of cytotoxicity. (A) 14 and 7 h broth culture supernatant fluids (20 μg of protein was loaded per lane) were treated with 80% (final concentration) ethanol and total, ethanol-insoluble, and ethanol-soluble fractions were separated by SDS-PAGE and Coomassie brilliant blue-stained. Arrow denotes small molecular weight (<10 kDa) band only present in total supernatant fluid or ethanol soluble fraction of FRI1169 culture (B) A549 cells were exposed to total, ethanol insoluble, and ethanol soluble fractions of FRI1169 and JY3000. Cytotoxicity was measured and plotted relative to media only (untreated) control. (^*Indicates P < 0.05 relative to media control).