Figure 5.

Sequence of Fos^RF. novicidaglpT vs. WT. DNA Sequencing of the entire ORF for glpT from F. novicida reveals nucleotide deletions and additions that result in missense frame shifts and stop codon insertion mutations from isolated colonies grown in the presence of fosmidomycin. GlpT transposon mutants are shown for comparison (Gallagher et al., 2007). (A) FnFos^R#1 – GC insertion at nt 401/402 results in frame shift after Try¹³³ and stop codon insert at nt 418/420. FnFos^R#2 – 14 bp deletions at nt 216 results in frame shift at Ala⁷² and stop codon insert at nt 238/240. FnFos^R#3 – G insertions at nt 517 results in frame shift after Val¹⁷¹ and stop codon insert at 541/543. FnFos^R#4 – 6 bp deletion at nt 1042 results in deletion of Ser³⁴⁶ and Leu³⁴⁷ from the alpha-helix H9. (B) Resulting amino acid alignment from the Fos^R mutants vs. WT F. novicida U112.