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. 2012 Mar 8;2:26. doi: 10.3389/fcimb.2012.00026

Figure 2.

Figure 2

SarA binds mRNA in vitro. (A) Gel mobility shift assays evaluating the mobility of DIG-labeled hla promoter DNA (Phla) in the presence of increasing amounts of purified SarA protein (0, 7.3, 36.6, 73.3, or 366.5 pmol). (B) Gel mobility shift assays evaluating the mobility of DIG-labeled, in vitro transcribed spa in the presence of increasing concentrations of SarA protein (0, 7.3, 36.6, 73.3, or 366.5 pmol). (C) Gel mobility shift assays evaluating the mobility of DIG-labeled, in vitro transcribed SSR42 in the presence of increasing concentrations of SarA protein (0, 36.6, 73.3, or 366.5 pmol). (D) Schematic of transcript variants containing deletions of 3′ or 5′ regions of spa that were DIG-labeled for use in subsequent gel-shift assays. (E) Gel mobility shift assays evaluating the mobility DIG-labeled spa deletion variants in the presence of 73.3 pmol SarA.