Table 2.

Oligonucleotides used in this study.

Primer name	Sequence (5′ → 3′)
RT-PCR
cna F	AACGAACAAGTATACACCAGGAGAG
cna R	TTTGCTTTTTCATCTAATCCTGTC
norA F	GCAGGTGCATTAGGCATTTTAGC
norA R	TGCCGATAAACCGAACGCTAAG
spa F	GCAGAAGCTAAAAAGCTAAATGATG
spa R	GCTCACTGAAGGATCGTCTTTAAGG
sarA F	TTTTAACCATGGCAATTACAAAAAT
sarA R	TTTCTCTTTGTTTTCGCTGATGTAT
PCR-MEDIATED IN VITRO TRANSCRIPTION*
L 1–450 spa T7 F	TAATACGACTCACTATAGGGCATACAGGGGGTATTAATTTGAAAA
L 1–450 spa R	ATCCTAGAATTCTCTTCGTTCAAGTTAGGCATGTTCA
L −250 spa T7 F	TAATACGACTCACTATAGGGGTTCAACAAAGATCAACAAAGCGCC
L −250 spa R	AGTAGAAAGTGTTGAGGCGTTTCAG
CLONING†,‡
c-Myc-SarA F	ATCCTAGTCGACGCTAACCCAGAAATACAATCACTGTGTC
c-Myc-SarA R	ATCCTAGGATCCTTACAGATCTTCTTCGCTGATCAGTTTCTGTTCTA GTTCAATTTCGTTGTTTGCTTCAGTG

*Bolded nucleotides indicate T7 promoter sequence.

^†Underlined nucleotides indicate restriction sites.

^‡Italicized nucleotides indicate the c-Myc epitope.