Table 4.
Total bacteria in the tracheobronchial lymph nodes (TBLN) and blooda.
| Time post-exposure | TBLN | Blood | ||
|---|---|---|---|---|
| # of animals | CFU | # of animals | CFU | |
| 1 Hour | ||||
| Sham-vaccinated | 4 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
| AVA-vaccinated | 4 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
| 6 Hour | ||||
| Sham-vaccinated | 5 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
| AVA-vaccinated | 5 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
| 12 Hour | ||||
| Sham-vaccinated | 5 | 44 ± 19 (3)b | 5 | 0.0 ± 0.0 |
| AVA-vaccinated | 5 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
| 24 Hour (experiment #1) | ||||
| Sham-vaccinated | 5 | 8785 ± 6397 (2) | 5 | 0.53 ± 0.32 (×106) (2) |
| AVA-vaccinated | 5 | 1315 ± 1139 (2) | 5 | 0.0 ± 0.0 |
| 24 Hour (experiment #2) | ||||
| Sham-vaccinated | 5 | NQc (3) | 5 | 0.33 ± 0.16 (×106) (3) |
| AVA-vaccinated | 5 | 0.0 ± 0.0 | 5 | 5600 ± 3542 (2) |
| 36 Hour (experiment #1) | ||||
| Sham-vaccinated | 3d | NQ (3) | 3 | 0.34 ± 0.11(×106) (3) |
| AVA-vaccinated | 5 | 95 ± 95 (1) | 5 | 0.0 ± 0.0 |
| 36 Hour (experiment #2) | ||||
| Sham-vaccinated | 4 | 277 ± 155 (×106) (4) | 5 | 18.69 ± 16.42 (×106) (4) |
| AVA-vaccinated | 5 | 0.0 ± 0.0 | 5 | 0.0 ± 0.0 |
a
Values are mean ± SEM.
b
Number in parenthesis are the number of animals testing positive (e.g., 3 of 5 sham-vaccinated animals had positive TBLNs at 12 h). However, for determining mean ± SEM, all data were used (zero's were included in the calculation).
c
NQ = not quantified; 3 of 5 lymph nodes were positive but bacteria were not quantified because plates were too numerous to count.
d
2 of 5 animals died prior to 36 h.