Fig. 3.

Internalization of chimeric receptor V1a/VRR1. Stably transfected HEK293 cells expressing chimeric receptor V1a/VRR1 were incubated in the absence (Left) or presence (Center and Right) of 10 μM AVP for 20 min at 37°C. The cells were fixed, permeabilized (Right) or nonpermeabilized (Left and Center), and then incubated with primary and secondary antibody (as described in Experimental Procedures). Samples were examined on a standard fluorescence microscope.