Figure 2.

CLSM Images of coverslip biofilms. LAC WT and LAC ΔsigB mutant biofilms were grown on human plasma-coated coverslips (A) or untreated coverslips (B). The biofilm were post-stained with SYTO9 (green) and TO-PRO3 (red), and a z series of images was obtained with CLSM to visualize biofilm capacity. Each image shows a larger top down (XY) view with cross-section views of biofilm depth on the right (YZ) and bottom (XZ). Only top down (XY) images were taken of LAC ΔsigB grown on plastic due to the lack of cell adherence. Biofilms did not form in the media only control wells (not shown).