Fig. 3.

Z-DNA-specific one-hybrid assay of Zα proteins. Yeast containing a LacZ reporter construct was transfected with vectors expressing Zα family proteins fused to an activation domain. (A) The results of quantitative assays for β-galactosidase are shown. Error bars are indicated. (B) When yeast is grown on plates containing 5-bromo-4-chloro-3-indolyl-β-d-galactoside, blue color indicates β-galactosidase synthesis due to binding of the protein to a stretch of Z-DNA near the promoter region of the LacZ reporter gene. Four colonies are shown for each construct. pACTA2 is transfected only with the vector.