Figure 2.
The Cia proteins are secreted from the C. jejuni F38011 wild-type strain and fliD and flaAflaB mutants, but not flgL, flgK, and flgE mutants. The C. jejuni F38011 wild-type strain and isogenic fliD, flaAB, flgL, flgK, and flgE mutants were assayed for secretion of the Cia proteins as well as for secretion of plasmid-encoded CiaC-ACD. Newly synthesized proteins were labeled with [35S]-methionine and Cia secretion was stimulated by the addition of 1% FBS. Proteins in the supernatants were concentrated 10-fold, separated by SDS-PAGE, transferred to PVDF membranes, and exposed to X-ray film (A) or probed with an anti-ACD antibody (B). Faint bands present in all lanes represent bovine serum albumin (66.5 kDa) and degradation products. The molecular mass of the pre-stained protein standards are listed in kDa. Supernatants (C) and whole-cell lysates (D) were probed with anti-CysM antibody to confirm that cell lysis did not occur.