Figure A3.

FlaG, FlaA, FlgB, and CiaI are secreted through the flagellar T3SS. Secretion of the FlaG-, FlaA-, FlgB-, CiaI-, and MetK-ACD fusion proteins from the C. jejuni wild-type strain and flgE mutant was examined. The C. jejuni strains were transformed with the various ACD plasmids, and Cia secretion was stimulated by the addition of FBS. After 3 h, the proteins in the supernatant fractions were precipitated and an immunoblot was performed using an anti-ACD (A) and anti-CysM (B) antibodies. MetK-ACD was included as a negative control, as it is not secreted from C. jejuni. The molecular mass of the pre-stained protein standards are listed in kDa.