Table 3.
Regulation of genes involved in DNA replication and repair, the cell cycle and apoptosis, following treatment of keratinocytes and HMEC-1 cells with CPF
| Process | Gene | HMEC-1 | Keratinocytes | |
|---|---|---|---|---|
|
| ||||
| 20 µg/mL | 20 µg/mL | 100 µg/mL | ||
| DNA replication | DNAPol ɛ | −2.29 | ||
| DNAPol α | −3.07 | |||
| PCNA | −1.51 | |||
| TOP2 | −1.56 | −1.81 | ||
| FEN1 | −2.09 | |||
| DNA ligase 1 | −1.92 | |||
| Cell cycle | Cyclin A | −2.59 | ||
| Cyclin B | −2.87 | |||
| Cyclin D | 1.61 | −2.05 | ||
| Cyclin E | ||||
| APC | 1.68 | −1.76 | −1.77 | |
| CDK1 [p34] | −1.51 | −2.35 | ||
| CDK2 | −1.86 | |||
| CDK4 | ||||
| CDK6 | 1.67 | |||
| E2F1 | −2.63 | |||
| E2F2 | −2.13 | |||
| DP1 | −1.87 | |||
| CDC25A | −2.03 | |||
| CDC25B | ||||
| CDC25C | −1.6 | |||
| Aurora A | −2.2 | |||
| Aurora B | −2.95 | |||
| p21 | 2.07 | 5.34 | ||
| GADD45α | 1.74 | 2.92 | ||
| GADD45β | 1.76 | −1.96 | −3.07 | |
| DNA repair | p53 | −1.83 | ||
| BRCA1 | −1.77 | |||
| ATM | ||||
| ATR | −1.57 | |||
| PARP-1 | −2.3 | |||
| Apoptosis | FasR (CD95) | 1.76 | ||
| Caspase 3 | −1.52 | |||
| Caspase 7 | −1.3 | |||
| BAX | 1.75 | |||
| BIM | 1.6 | |||
| PUMA | 3.56 | |||
| BIK | 5.57 | |||
| BCL-XL | 1.7 | |||
| NOXA | −1.78 | 2.66 | ||