Fig. 7.
ERβ regulates cyclin E expression at mRNA and protein levels. (a) Real-time PCR on cyclin E1 mRNA in T47D cells treated with 10 nM E2 for different times. (b) Western blot using antibody to cyclin E on extracts from T47D cells treated with E2 for 24 h compared to nontreated control. (c) Transient transfection of T47D cells with cyclin E promoter luciferase construct ± transfected pcDNA3 FLAG ERβ.